Microbiota Trace in Blood Patients with Rheumatoid arthritis

Introduction and Aim: In recent years the relationship of RA and microbiome has received a lot of attention. There are several reports on gut microbiota, Oral Microbiota and dysbiosis of microbiota. While, the blood microbiota has not considered yet. The purpose of this study was to assay the blood microbiome in RA patients.
methods: the blood samples were taken from 102 patients with RA. The samples were subjected to DNA extraction. Based on gene bank data, the reference sequences for 16s RNA, nuc and rfbE genes were used. Then, the primer pairs for replication of these genes fragments were designed, and bioinformatics characteristic were assayed. Using synthesized primers and templet genome PCR amplification was performed. By electrophoresis and observation of amplicons fragments, sequencing was determined.
Results: Seventy-five percent (75%) of the sample were amplified the fragment of the 16sRNA gene and confirmed by sequencing. Furthermore, in 54% of the samples, the nuc gene and in 10% of samples the rfbE gene were detected respectively. The results were analyzed and the relation was significates.
Conclusion: Although, more research is needed. the result of the study is very important, because in general the universal genome of at least 10 bacterial species were traced, and specifically in 54% of the samples, the Staphylococcal species and in 10% cases the Escherichia species were traceable. Anyway, though the mechanism of triggering the RA not known by these bacteria, these data may be useful for better management and control of RA disease.